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syntaxin3  (Danaher Inc)


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    Danaher Inc syntaxin3
    Syntaxin3, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/syntaxin3/10__7554_slash_elife__80135-233-112-118
    Average 86 stars, based on 1 article reviews
    syntaxin3 - by Bioz Stars, 2026-10
    86/100 stars

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    Western Blot:

    Article Title: A CRISPR screen in intestinal epithelial cells identifies novel factors for polarity and apical transport
    Article Snippet: .. The following antibodies were commercially obtained and used as stated: Aminopeptidase N (IF 1:50, #HBB3- 153- 63, DSHB), Anoctamin 8 (WB 1:500, #HPA049206, Atlas Antibodies), ARHGAP33 (Western blotting [WB] 1:500, #HPA030117, Atlas Antibodies), beta- Actin (WB 1:2000, #A2228, Sigma- Aldrich), cathepsin D (EM 1:50, #219361, Calbiochem), CD63/Lamp3 (EM 1:20, #M1544, Sanquin), Claudin 3 (IF 1:200, #SAB4500435, Sigma- Aldrich), DNP (EM 1:6- 1:30, #71- 3500, Invitrogen), DPP4 (IF/FACS 1:100, #HBB3/775/42, DSHB; EM 1:10, #AF1180, R&D Systems), E- cadherin (IF 1:200, EM 1:30, #610181, BD Bioscences), GALNT2 (WB 1:1000, #AF7507, Novus Biologicals), Lamp1 (IF 1:200, EM 1:10, #1D4B, DSHB), MTMR2 (WB 1:500, #sc- 365184, Santa Cruz Biotechnology), PIP5K1C (WB 1:1000, #3296S, Cell Signaling Technology), Syntaxin3 (IF 1:100, EM 1:10, #133750, Abcam), sucrase- isomaltase (IF 1:100, #HBB2/614/88, DSHB), TM9SF4 (WB 1:1000, #sc- 374473, Santa Cruz Biotechnology), TMEM110 (WB 1:1000, #NBP1- 69238, Novus Biologicals), and ZO- 1 (EM 1:50, #61- 7300 Zymed). .. Secondary horseradish peroxidase- coupled (HRP) goat anti- mouse and goat anti- rabbit (1:5000, Sigma- Aldrich) were used for WB, and secondary Alexa Fluor- conjugated (Alexa Fluor 488 and 568) goat anti- mouse (1:1000, Life Technologies) and goat anti- rabbit (1:1000, Life Technologies) were used for IF labeling.



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    Fig. 4. Effects of SKF-10047 on the protein expression of <t>Syntaxin3</t> (STX3). Syntaxin3 consists of some isoforms, produced by alternative splicing (https://www.uniprot. org/uniprot/Q13277). According to the predictive molecular size of isoforms, the isoform A and isoform B, main isoforms of STX3 were considered to be detected in COS-7 cells (left). Treatment of COS-7 cells with SKF-10047 for 48 h significantly increased the protein levels of both STX3 isoforms in a dose-dependent manner. Statistical analysis was performed for isoform B (*P < 0.05, n ¼ 3, one-way ANOVA with Dunnett's post-test).
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    Fig. 4. Effects of SKF-10047 on the protein expression of <t>Syntaxin3</t> (STX3). Syntaxin3 consists of some isoforms, produced by alternative splicing (https://www.uniprot. org/uniprot/Q13277). According to the predictive molecular size of isoforms, the isoform A and isoform B, main isoforms of STX3 were considered to be detected in COS-7 cells (left). Treatment of COS-7 cells with SKF-10047 for 48 h significantly increased the protein levels of both STX3 isoforms in a dose-dependent manner. Statistical analysis was performed for isoform B (*P < 0.05, n ¼ 3, one-way ANOVA with Dunnett's post-test).
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    Fig. 4. Effects of SKF-10047 on the protein expression of <t>Syntaxin3</t> (STX3). Syntaxin3 consists of some isoforms, produced by alternative splicing (https://www.uniprot. org/uniprot/Q13277). According to the predictive molecular size of isoforms, the isoform A and isoform B, main isoforms of STX3 were considered to be detected in COS-7 cells (left). Treatment of COS-7 cells with SKF-10047 for 48 h significantly increased the protein levels of both STX3 isoforms in a dose-dependent manner. Statistical analysis was performed for isoform B (*P < 0.05, n ¼ 3, one-way ANOVA with Dunnett's post-test).
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    Fig. 4. Effects of SKF-10047 on the protein expression of <t>Syntaxin3</t> (STX3). Syntaxin3 consists of some isoforms, produced by alternative splicing (https://www.uniprot. org/uniprot/Q13277). According to the predictive molecular size of isoforms, the isoform A and isoform B, main isoforms of STX3 were considered to be detected in COS-7 cells (left). Treatment of COS-7 cells with SKF-10047 for 48 h significantly increased the protein levels of both STX3 isoforms in a dose-dependent manner. Statistical analysis was performed for isoform B (*P < 0.05, n ¼ 3, one-way ANOVA with Dunnett's post-test).
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    Fig. 4. Effects of SKF-10047 on the protein expression of Syntaxin3 (STX3). Syntaxin3 consists of some isoforms, produced by alternative splicing (https://www.uniprot. org/uniprot/Q13277). According to the predictive molecular size of isoforms, the isoform A and isoform B, main isoforms of STX3 were considered to be detected in COS-7 cells (left). Treatment of COS-7 cells with SKF-10047 for 48 h significantly increased the protein levels of both STX3 isoforms in a dose-dependent manner. Statistical analysis was performed for isoform B (*P < 0.05, n ¼ 3, one-way ANOVA with Dunnett's post-test).

    Journal: Journal of pharmacological sciences

    Article Title: SKF-10047, a prototype Sigma-1 receptor agonist, augmented the membrane trafficking and uptake activity of the serotonin transporter and its C-terminus-deleted mutant via a Sigma-1 receptor-independent mechanism.

    doi: 10.1016/j.jphs.2018.11.005

    Figure Lengend Snippet: Fig. 4. Effects of SKF-10047 on the protein expression of Syntaxin3 (STX3). Syntaxin3 consists of some isoforms, produced by alternative splicing (https://www.uniprot. org/uniprot/Q13277). According to the predictive molecular size of isoforms, the isoform A and isoform B, main isoforms of STX3 were considered to be detected in COS-7 cells (left). Treatment of COS-7 cells with SKF-10047 for 48 h significantly increased the protein levels of both STX3 isoforms in a dose-dependent manner. Statistical analysis was performed for isoform B (*P < 0.05, n ¼ 3, one-way ANOVA with Dunnett's post-test).

    Article Snippet: An anti-Syntaxin3 rabbit polyclonal antibody was purchased from Alomone labs. (Jerusalem, Israel).

    Techniques: Expressing, Produced, Alternative Splicing

    Fig. 5. Influence of knockdown or overexpression of Syntaxin3 on the incremental effects of SKF-10047 on the SERTDCT uptake activity. We examined the effects of SKF-10047 on the SERTDCT uptake activity in STX3-knockdown or STX3-overexpressing COS-7 cells, and compared the increasing rate in these cells to that in control cells. A: Validation of STX3 knockdown by STX3 siRNA (upper) and overexpression by pMyc-DDK-STX3 (lower) in COS-7 cells. The transfection of STX3 siRNA #1 and #2 reduced the expression of STX3 isoform A, a functional isoform, by 53% and 56%, respectively (upper). In pMyc-DDK-STX3-transfected cells, prominent expression of Myc-DDK-STX3 was seen in addition to endogenous STX3, isoform A and B. B: Effects of SKF-10047 on the uptake activity of SERTDCT in control siRNA-transfected COS-7 cells and STX3 siRNA #2-transfected COS-7 cells. In both cells, 200 mM SKF-10047 significantly increased the uptake activity of SERTDCT (n ¼ 4, P < 0.001, unpaired t-test). The increase in SERTDCT uptake activity induced by 200 mM SKF-10047 was similar in control siRNA-transfected cells and STX3 siRNA-transfected cells (n ¼ 4, ns, unpaired t-test). C: Effects of SKF-10047 on the uptake activity of SERTDCT in mock-transfected COS-7 cells and Myc-DDK-STX3-expressing COS-7 cells. In both cells, 200 mM SKF-10047 significantly increased the uptake activity of SERTDCT (n ¼ 4, P < 0.001, unpaired t-test). The increase in SERTDCT uptake activity induced by 200 mM SKF-10047 in Myc-DDK-STX3-expressing cells was significantly higher than that in mock-transfected cells (n ¼ 4, P < 0.001, unpaired t-test).

    Journal: Journal of pharmacological sciences

    Article Title: SKF-10047, a prototype Sigma-1 receptor agonist, augmented the membrane trafficking and uptake activity of the serotonin transporter and its C-terminus-deleted mutant via a Sigma-1 receptor-independent mechanism.

    doi: 10.1016/j.jphs.2018.11.005

    Figure Lengend Snippet: Fig. 5. Influence of knockdown or overexpression of Syntaxin3 on the incremental effects of SKF-10047 on the SERTDCT uptake activity. We examined the effects of SKF-10047 on the SERTDCT uptake activity in STX3-knockdown or STX3-overexpressing COS-7 cells, and compared the increasing rate in these cells to that in control cells. A: Validation of STX3 knockdown by STX3 siRNA (upper) and overexpression by pMyc-DDK-STX3 (lower) in COS-7 cells. The transfection of STX3 siRNA #1 and #2 reduced the expression of STX3 isoform A, a functional isoform, by 53% and 56%, respectively (upper). In pMyc-DDK-STX3-transfected cells, prominent expression of Myc-DDK-STX3 was seen in addition to endogenous STX3, isoform A and B. B: Effects of SKF-10047 on the uptake activity of SERTDCT in control siRNA-transfected COS-7 cells and STX3 siRNA #2-transfected COS-7 cells. In both cells, 200 mM SKF-10047 significantly increased the uptake activity of SERTDCT (n ¼ 4, P < 0.001, unpaired t-test). The increase in SERTDCT uptake activity induced by 200 mM SKF-10047 was similar in control siRNA-transfected cells and STX3 siRNA-transfected cells (n ¼ 4, ns, unpaired t-test). C: Effects of SKF-10047 on the uptake activity of SERTDCT in mock-transfected COS-7 cells and Myc-DDK-STX3-expressing COS-7 cells. In both cells, 200 mM SKF-10047 significantly increased the uptake activity of SERTDCT (n ¼ 4, P < 0.001, unpaired t-test). The increase in SERTDCT uptake activity induced by 200 mM SKF-10047 in Myc-DDK-STX3-expressing cells was significantly higher than that in mock-transfected cells (n ¼ 4, P < 0.001, unpaired t-test).

    Article Snippet: An anti-Syntaxin3 rabbit polyclonal antibody was purchased from Alomone labs. (Jerusalem, Israel).

    Techniques: Knockdown, Over Expression, Activity Assay, Control, Biomarker Discovery, Transfection, Expressing, Functional Assay